anti phospho mtor  (Cell Signaling Technology Inc)

Journal: Molecular Medicine Reports

Article Title: Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway

doi: 10.3892/mmr.2025.13545

Figure Lengend Snippet: Antcin K suppresses PI3K, Akt and mTOR signaling. Western blotting analysis of levels of p-p85, Akt and mTOR after 24 h incubation with antcin K. *P<0.05 vs. control. p-, phosphorylated.

Article Snippet: The phosphorylated (p)-p85 (1:2,000; cat. no. 4228S), p-Akt (1:2,000; cat. no. 4060S), p-mTOR (1:2,000; cat. no. 5536S), mTOR (1:2,000; cat. no. 2983S) and p-p65 (1:2,000; cat. no. 3033) antibodies were obtained from Cell Signaling Technology, Inc. Antibodies for MMP-7 (1:500; cat. no. sc-515703), Akt (1:500; cat. no. sc-5298) and p85 (1:500; cat. no. sc-1637) were purchased from Santa Cruz Biotechnology, Inc. p65 (1:2,000; cat. no. GTX102090) and β-actin (1:5,000; cat. no. GT5512) antibodies were purchased from GeneTex International Corporation.

Techniques: Western Blot, Incubation, Control

Journal: Molecular Medicine Reports

Article Title: Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway

doi: 10.3892/mmr.2025.13545

Figure Lengend Snippet: Inhibitory effect of antcin K on chondrosarcoma cell motility. Antcin K inhibits chondrosarcoma cell migration and invasion by decreasing MMP-7 expression by downregulating PI3K, Akt, mTOR and NF-κB signaling cascades. Image created using BioRender.com. p-, phosphorylated.

Article Snippet: The phosphorylated (p)-p85 (1:2,000; cat. no. 4228S), p-Akt (1:2,000; cat. no. 4060S), p-mTOR (1:2,000; cat. no. 5536S), mTOR (1:2,000; cat. no. 2983S) and p-p65 (1:2,000; cat. no. 3033) antibodies were obtained from Cell Signaling Technology, Inc. Antibodies for MMP-7 (1:500; cat. no. sc-515703), Akt (1:500; cat. no. sc-5298) and p85 (1:500; cat. no. sc-1637) were purchased from Santa Cruz Biotechnology, Inc. p65 (1:2,000; cat. no. GTX102090) and β-actin (1:5,000; cat. no. GT5512) antibodies were purchased from GeneTex International Corporation.

Techniques: Migration, Expressing