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rabbit anti p mtor  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti p mtor
    Rabbit Anti P Mtor, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+mtor/pm41763584-177-48-77
    Average 86 stars, based on 1 article reviews
    rabbit anti p mtor - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: iPSCNPC transplantation alleviates brain injury after intracerebral hemorrhage in mice by downregulating autophagy via AMPK/ mTOR signaling pathway.
    Article Snippet: .. Immunofluorescence Immunofluorescence staining was performed on brain cryosections and astrocyte cultures as previously described.30,33 The following primary antibodies were used: rabbit anti-AMPK (1:100, CST), rabbit anti-mTOR (1:100, CST), mouse anti-GFAP (1:1000, Servicebio), rabbit anti-GFAP (1:1000, Servicebio), and mouse anti-VIM (1:500, Servicebio). .. Corresponding secondary antibodies (1:500, Beyotime) included Alexa Fluor 488-conjugated goat anti-rabbit IgG, Alexa Fluor 488-conjugated goat anti-mouse IgG, Alexa Fluor 555-conjugated donkey anti-mouse IgG, Alexa Fluor 555-conjugated donkey anti-rabbit IgG, and Alexa Fluor 647-conjugated goat anti-mouse IgG.

    Staining:

    Article Title: iPSCNPC transplantation alleviates brain injury after intracerebral hemorrhage in mice by downregulating autophagy via AMPK/ mTOR signaling pathway.
    Article Snippet: .. Immunofluorescence Immunofluorescence staining was performed on brain cryosections and astrocyte cultures as previously described.30,33 The following primary antibodies were used: rabbit anti-AMPK (1:100, CST), rabbit anti-mTOR (1:100, CST), mouse anti-GFAP (1:1000, Servicebio), rabbit anti-GFAP (1:1000, Servicebio), and mouse anti-VIM (1:500, Servicebio). .. Corresponding secondary antibodies (1:500, Beyotime) included Alexa Fluor 488-conjugated goat anti-rabbit IgG, Alexa Fluor 488-conjugated goat anti-mouse IgG, Alexa Fluor 555-conjugated donkey anti-mouse IgG, Alexa Fluor 555-conjugated donkey anti-rabbit IgG, and Alexa Fluor 647-conjugated goat anti-mouse IgG.

    Western Blot:

    Article Title: iPSCNPC transplantation alleviates brain injury after intracerebral hemorrhage in mice by downregulating autophagy via AMPK/ mTOR signaling pathway.
    Article Snippet: .. Protein extracts from brain tissues and astrocytes were prepared according to experimental requirements, and Western blotting was conducted as previously reported.33 Primary antibodies included: rabbit anti-AMPK (1:1000, CST), rabbit anti-phospho-AMPK (1:1000, CST), rabbit anti-mTOR (1:1000, CST), rabbit anti-phospho-mTOR (1:1000, CST), mouse anti-P62 (1:1000, Abcam), rabbit anti-LC3 (1:1000, CST), mouse anti-GFAP (1:1000, Servicebio), mouse anti-VIM (1:1000, Servicebio), and mouse anti-GAPDH (1:1000, Servicebio). .. Signals were detected using an enhanced chemiluminescence substrate (ThermoFisher Scientific) and imaged with a Tanon imaging system (Shanghai, China).

    Incubation:

    Article Title: Edaravone dexborneol promotes oligodendrocyte precursor cell proliferation and differentiation to repair white matter and improve long-term outcomes after ischemic stroke.
    Article Snippet: Then, 25 μg of protein was subjected to electrophoresis using 10–12% SDS polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (cat. no. 10600021; GE Healthcare). .. The membranes were blocked with 10% non-fat milk in Tween/Tris-buffered salt solution (20 mM Tris-Cl pH 7.5, 0.15 M NaCl, 0.05% Tween-20) for 1 h, then incubated overnight at 4̊C with rabbit anti-Akt (cat. no. 4691), rabbit anti-phosphorylated (p-)Akt (Thr308; cat. no. 13038), rabbit anti-mTOR (cat. no. 2983), rabbit anti-p-mTOR (Ser2481; cat. no. 2974), rabbit anti-S6 ribosomal protein (cat. no. 2217), rabbit anti-p-S6 (Ser235/236; cat. no. 4858) and rabbit anti-GAPDH (cat. no. 2118) primary antibodies (all from CST Biological Reagents Co., Ltd. and used at a dilution of 1:1000). .. After washing for 30 min, the PVDF membranes were incubated with the horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (Abcam; cat. no. ab6721; 1:10,000) at room temperature for 1.5 h. Reactivity was visualized using an enhanced chemiluminescence (ECL) kit (MilliporeSigma) and captured with a Bio-Rad ECL detection system.

    Article Title: Multiple mTOR RNA localization signals regulate subcellular protein synthesis and axonal growth
    Article Snippet: .. The cells were then incubated with rabbit anti-mTOR (Cell Signaling cat. 2983S, 1:100) and mouse anti-puromycin (1:5,000) antibodies in blocking buffer overnight at 4°C. .. The proximity ligation assay was done using the Duolink kit (Sigma: PLA probe anti-mouse minus DUO92004, anti-rabbit plus DUO92002, with detection using Far-Red DUO92013) according to the manufacturer’s protocol.

    Concentration Assay:

    Article Title: Ribonuclease 4 Functions in Nociceptor-Mediated Nerve Homeostasis
    Article Snippet: Excitation was provided by Coherent OBIS lasers at 488 nm, 561 nm, and 640 nm with appropriate emission filters. .. The following antibodies were applied overnight at 4 °C at a concentration of 1:1000: rabbit anti-Axl (CST, 8661), rabbit anti-phospho-Axl Tyr702 (CST, 5724), rabbit anti-mTOR (CST, 2983), rabbit anti-phospho-mTOR Ser2448 (CST, 5536), rabbit anti-Akt (CST, 9271), rabbit anti-phospho-Akt (CST, 9272), and mouse anti-βIII tubulin (Promega, G712A). ..

    other:

    Article Title: Ribonuclease 4 Functions in Nociceptor-Mediated Nerve Homeostasis.
    Article Snippet: were processed with 10mM dithiothreitol, and NuPAGE LDS Sample Buffer (ThermoFisher, NP0007).

    Blocking Assay:

    Article Title: Multiple mTOR RNA localization signals regulate subcellular protein synthesis and axonal growth
    Article Snippet: .. The cells were then incubated with rabbit anti-mTOR (Cell Signaling cat. 2983S, 1:100) and mouse anti-puromycin (1:5,000) antibodies in blocking buffer overnight at 4°C. .. The proximity ligation assay was done using the Duolink kit (Sigma: PLA probe anti-mouse minus DUO92004, anti-rabbit plus DUO92002, with detection using Far-Red DUO92013) according to the manufacturer’s protocol.



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